ANTI-PROLIFERATIVE EFFICACY OF ALSTONIA SCHOLARIS (L.) R.BR. EXTRACT ON CULTURED DAUDI CELLS: AN IN VITRO APPROACH
Keywords:Antiproliferation, apoptosis, Daudi cell line, Alstonia scholaris
Objective: The aim of this study is to observe the apoptosis of ALSTONIA SCHOLARIS (L.) R.Br. against Daudi cells and to study its primary mechanism.
Materials and Methods: Antiproliferative activity of cultured Daudi cells was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay in a dose- and time-dependent manner after treatment with the hydroalcoholic extract of A. scholaris. Trypan blue viability assay was also performed. Apoptosis induction in the cells post treatment was determined by DNA fragmentation assay, Agarose gel electrophoresis, and Acridine orange/Ethidium bromide dual staining. Protein isolation and analysis was carried out using the standard polyacrylamide gel electrophoresis protocols.
Results: The extracts inhibited the growth and proliferation of Daudi cells through induced cell death, which was dose-dependent and time-dependent. The IC50 value was found to be 260 µg/ml after 72 h of treatment. The induction of DNA fragmentation and increase in a number of apoptotic ells post treatment suggest the possibility of apoptosis induction. A significant decrease in protein level was also observed.
Conclusion: The results raise the possibility that the hydroalcoholic extract of A. scholaris could be a potent chemotherapeutic agent for the treatment of various cancers. Further evaluation of its potency as a chemotherapeutic agent is imperative.
World Health Organization. Cancer control: knowledge into action: WHO guide for effective programmes. Geneva: WHO; 2007.
American Cancer Society. Cancer Facts & Figures. Atlanta, GA: American Cancer Society. 2016
Dewi M. The distribution of cancer in Indonesia, basic health research in 2007. Indonesian J Cancer 2017;11(1):1-2.
Mukherjee T. Varied clinical presentation of non-Hodgkin lymphoma. Clin Med 2016; 2(2):25-30.
Epstein MA, Achong BG and Barr YM. Virus particles in cultured lymphoblasts from burkitt’s lymphoma. The Lancet 1964;283(7335):702-703.
Schulz TF, Boshoff CH, Weiss RA. HIV infection and neoplasia. The Lancet 1996;348(9027):587-591.
Manolov G and Manolova Y. Marker band in one chromosome 14 from Burkitt lymphomas. Nature 1972; 237(5349): 33-34.
Zech L, Haglund U, Nilsson K and Klein G. Characteristic chromosomal abnormalities in biopsies and lymphoid-cell lines from patients with burkitt and non-burkitt lymphomas. Int J of Cancer 1976; 17(1): 47-56.
Burkitt DP. Etiology of Burkitt's lymphoma—an alternative hypothesis to a vectored virus. J N Cancer Institute 1969; 42(1): 19-28.
Parmar F, Kushwah N, Highland H, George LB. In vitro antioxidant and anticancer activity of Mimosa pudica Linn extract and L-Mimosine on lymphoma Daudi cells. Int J Pharm Pharma Sci 2015;7:100-4.
Parmar F, Patel C, Highland H, Pandya H and George LB. Antiproliferative efficacy of Kaempferol on culture Daudi cells: An in silico and in vitro study. Adv Biol 2016; 2016:1-10.
Harbone JB. Phytochemical methods: A Guide to Modern Techniques of plant Analysis. London: Chapman and Hall 1973 pp. 49-188.
Phillips HJ and Terryberry JE. Counting actively metabolizing tissue cultured cells. Exp Cell Res 1957;13:341-7.
Kuttan R, Bhanumathly P, Nirmala K and George MC. Potential anticancer activity of turmeric (Curcuma longa). Cancer lett 1985;29:197-202.
Mosmann T. Rapid Colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol methods 1983;65:55-63.
Wilson AP. Cytotoxicity and viability assay in animal cell culture, A practical approach. 3rd ed. New York 2000 pp. 175-219.
Chomczynski P, Mackey K, Drews R, Wilfinger W. DNAzol®: A reagent for the rapid isolation of genomic DNA. BioTechniques 1997;22:550-553.
Jimenez PC, Wilke DV, Takeara R, Lotufo TMC, Pessoa C, de Moraes MO. Cytotoxic activity of a dichloromethane extract and fractions obtained from Eudistoma vannamei (Tunicata: Ascidiacea). Comp Biochem Physiol Part A 2008;151:391-8.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.
Wang C, Chen K, Wu S, Jhan Y and Shyu C. Anti-Proliferative activity of triterpenoids and sterols isolated from Alstonia scholaris against non-small- cell lung carcinoma cells. Molecules 2017;22:1-13.
Bagheri G, Mirzaei M, Mehrabi R and Sharifi-Rad J. Cytotoxic and antioxidant activities of Alstonia scholaris, Alstonia venenata and Moringa oleifera from India. Jundishapur J Nat Pharm Prod, 2016;11(3):e31129.
Feng L, Chen Y, Yuan L, Liu X, Gu J, Zhang M and Wang Y. A combination of Alkaloids and Triterpenes of Alstonia scholaris (Linn) R. Br. Leaves enhances immunomodulatory activity in C57BL/6 mice and induces apoptosis in the A549 cell line. Molecules 2013; 18:13920-13939.
Parmar F, George LB and Highland H. Phyllanthus fraternus manifests potent anti-proliferative activity on cultured Daudi cells. J cancer res 2018;1-8.
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